|
KMID : 0644120130260020017
|
|
Journal of Oriental Gynecology
2013 Volume.26 No. 2 p.17 ~ p.32
|
|
|
A Study on the Inhibitory Effect and Mechanism of Lonicera Japonica on Type Ⅰ Interferon
|
|
Kang Yong-Goo
Ryu Ik-Han
Kim Song-Baek
Choi Chang-Min
Seo Yun-Jung
Cho Han-Baek
|
|
|
Abstract
|
|
|
Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type Ⅰ IFN production.
Methods: To evaluate inhibitory effect of LJ on type Ⅰ IFN, we examined type Ⅰ IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot.
Results: LJ inhibited Type Ⅰ IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type Ⅰ IFN and IRF-1, 7 than LPS-induced LJ pretreatment.
Conclusions: LJ inhibits LPS-induced production of type Ⅰ IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.
|
|
|
KEYWORD
|
|
|
Lonicera japonica, Type Ⅰ interferon(IFN), Interleukin-10(IL-10), Interferon regulatory factor(IRF), Signal transducers and activators of transcription(STAT)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|